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F9880

Sigma-Aldrich

Fluorinert™ FC-70

Synonym(s):

Perfluorotriamylamine, fluorocarbon FC 70

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About This Item

CAS Number:
338-84-1
EC Number:
206-421-8
MDL number:
MFCD00042367
UNSPSC Code:
15121530
PubChem Substance ID:
24895003
NACRES:
NA.28

description

used in density gradient studies

Quality Level

100

density

1.93 g/mL at 25 °C (lit.)

SMILES string

FC(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)N(C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F

InChI

1S/C15F33N/c16-1(17,4(22,23)10(34,35)36)7(28,29)13(43,44)49(14(45,46)8(30,31)2(18,19)5(24,25)11(37,38)39)15(47,48)9(32,33)3(20,21)6(26,27)12(40,41)42

InChI key

AQZYBQIAUSKCCS-UHFFFAOYSA-N

Application

Fluorinert™ FC-70 has been used to reduce the adherence of large trypsin:N-amidinopiperidine crystals to the well. It has also been used as a fluorocarbon oil for microinjection of oocytes.
For use as a high density chase solution in density gradient studies.

Packaging

Packaged and distributed by Sigma-Aldrich

Other Notes

Fluorinert FC-70 is a liquid mixture of completely fluorinated aliphatic compounds.

Legal Information

Fluorinert is a trademark of 3M Company

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Quantitative microinjection of oocytes, eggs, and embryos
Jaffe LA and Terasaki M
Methods in Cell Biology, 74, 219-242 (2004)
Microinjection: Methods and Applications (2016)
Intriguing role of water in protein-ligand binding studied by neutron crystallography on trypsin complexes
Schiebel J, et al.
Nature Communications, 9 (2018)
Alexandra Colin et al.
The Journal of cell biology, 219(3) (2020-01-18)
Nucleus centering in mouse oocytes results from a gradient of actin-positive vesicle activity and is essential for developmental success. Here, we analyze 3D model simulations to demonstrate how a gradient in the persistence of actin-positive vesicles can center objects of

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