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Key Documents

D2813

Sigma-Aldrich

4,5-Diaminofluorescein diacetate

≥98% purity (HPLC), lyophilized powder

Synonym(s):

DAF-2 DA

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About This Item

Empirical Formula (Hill Notation):
C24H18N2O7
CAS Number:
Molecular Weight:
446.41
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

4,5-Diaminofluorescein diacetate, ≥98% (HPLC)

Quality Level

Assay

≥98% (HPLC)

form

lyophilized powder

color

colorless to white

solubility

DMSO: <2 mg/mL
H2O: <2 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

shipped in

wet ice

storage temp.

2-8°C

SMILES string

O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=C(N)C(N)=C5

InChI

1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

InChI key

PTSUYDXEEKDBQU-UHFFFAOYSA-N

Application

4,5-Diaminofluorescein diacetate (DAF-2 DA) has been used as a fluorescent dye for NO (nitric oxide) detection.
Cell permeable fluorescent detector of nitric oxide in living cells.

Legal Information

DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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