D2429

DMEM - low glucose

With sodium pyruvate, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable fore cell culture

• Synonym(s): DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
• MDL number: MFCD00217342
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Dulbecco′s Modified Eagle′s Medium - low glucose, 10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture
• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• concentration: 10 ×
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: no; sodium pyruvate: yes; phenol red: yes; glucose: low; L-glutamine: no; HEPES: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of basal medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose.

Application

Dulbecco′s Modified Eagle′s Medium-low glucose has been used:

• during organoid embedding for neutralizing thawed collagen I
• to culture melanoma cancer cell lines
• to store the cancer tissue samples
• as a differentiation culture medium for C2C12 cells

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate, 0.584 g/L L-glutamine, 0.004 g/L folic acid, at 1×.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable