CLLS1098
SW48 Cells HIF1A -/-/-
Human female colon, colorectal adenocarcinoma
Synonym(s):
CLL1008
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DKK 2,120.00
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About This Item
DKK 2,120.00
Availability
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product name
SW48 Cells HIF1A -/-/-,
biological source
human female colon (Source disease: Colorectal adenocarcinoma)
Quality Level
storage temp.
−196°C
Gene Information
human ... HIF1A(3091)
1 of 4
This Item | CLLS1007 | CLLS1009 | CLLS1006 |
---|---|---|---|
−196°C | −196°C | −196°C | −196°C |
human ... HIF1A(3091) | human ... TP53(7157) | human ... PTEN(5728) | human ... BAX(581) |
200 | 200 | 200 | 200 |
General description
This product corresponds to ATCC Cat. No. CCL-231.
Application
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture ratio: approx. 1:2 to 1:6.
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Cat. No. S8636, to a final concentration of 1 mM.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
Features and Benefits
Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2
Quality
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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