C8571

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

• Synonym(s): Cathepsin B₁
• CAS Number: 9047-22-7
• Enzyme Commission number: 3.4.22.1 (BRENDA, IUBMB)
• MDL number: MFCD00130748
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human liver
• Quality Level: 200
• form: buffered aqueous solution
• specific activity: ≥1,500 units/mg protein (E1%/280)
• UniProt accession no.: P07858
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CTSB(1508)

Description

General description

Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.

Application

Cathepsin B from human liver has been used:

• as a positive control in the screening Cathepsin B inhibitor assay
• in wall extension (expansin activity) assay
• in microinjection human foreskin fibroblasts to test its effect on apoptosis induction

Biochem/physiol Actions

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Packaging

Package size based on protein content

Unit Definition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Physical form

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable