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C1535

Sigma-Aldrich

Cibacron Blue 3GA Agarose

Type 3000-CL, saline suspension

Synonym(s):

1-amino-4-[4-[[4-chloro-6-(2-sulfoanilino)-1,3,5-triazin-2-yl]amino]-3-sulfoanilino]-9,10-dioxoanthracene-2-sulfonic acid

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About This Item

MDL number:
MFCD00043102
UNSPSC Code:
23151817
PubChem Substance ID:
24892421
NACRES:
NA.56

biological source

plant

Quality Level

200

type

Type 3000-CL

form

saline suspension

extent of labeling

2-6 μmol per per mL

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

capacity

≥5 mg/mL binding capacity (bovine serum albumin)

suitability

suitable for chromatography

storage temp.

2-8°C

SMILES string

[Na+].[Na+].[Na+].Nc1c(cc(Nc2ccc(Nc3nc(Cl)nc(Nc4cccc(c4)S([O-])(=O)=O)n3)c(c2)S([O-])(=O)=O)c5C(=O)c6ccccc6C(=O)c15)S([O-])(=O)=O

InChI

1S/C29H20ClN7O11S3.3Na/c30-27-35-28(33-13-4-3-5-15(10-13)49(40,41)42)37-29(36-27)34-18-9-8-14(11-20(18)50(43,44)45)32-19-12-21(51(46,47)48)24(31)23-22(19)25(38)16-6-1-2-7-17(16)26(23)39;;;/h1-12,32H,31H2,(H,40,41,42)(H,43,44,45)(H,46,47,48)(H2,33,34,35,36,37);;;/q;3*+1/p-3

InChI key

VZPXDCIISFTYOM-UHFFFAOYSA-K

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Application

Cibacron Blue 3GA-agarose is an agarose conjugate in saline suspension used in affinity chromatography, protein chromatography and dye resins.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Johanna E Simkin et al.
Developmental biology, 446(1), 22-33 (2018-11-19)
The enteric nervous system is mostly derived from vagal neural crest (NC) cells adjacent to somites (s)1-7. We used in ovo focal fluorescent vital dyes and focal electroporation of fluorophore-encoding plasmids in quail embryos to investigate NC cell migration to
María del Pilar Ferraris et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(26), 2741-2745 (2011-08-30)
The objective of this study was the development of affinity adsorbent particles with the appropriate characteristics to be applied in protein purification using the affinity ultrafiltration method. To prepare affinity macroligands Cibacron Blue 3GA, as a ligand molecule, was immobilized
Takashi Miura et al.
Free radical biology & medicine, 51(11), 2082-2089 (2011-10-04)
1,2-Naphthoquinone (1,2-NQ) is electrophilic, and forms covalent bonds with protein thiols, but its two-electron reduction product 1,2-dihydroxynaphthalene (1,2-NQH(2)) is not, so enzymes catalyzing the reduction with reduced pyridine nucleotides as cofactors could protect cells from electrophile-based chemical insults. To assess
Richa Gupta et al.
Molecular biology reports, 39(5), 5607-5614 (2011-12-23)
MspNI and MspNII, isoschizomers of prototype Type II restriction endonucleases AvaII and BstYI, were extracted from an extreme thermophile bacterium belonging to the genus Meiothermus, isolated from the hot sulphur springs in north Himalayan region of India where temperature and
Andreas Brunschweiger et al.
Journal of medicinal chemistry, 51(15), 4518-4528 (2008-07-18)
Ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDases, subtypes 1, 2, 3, 8 of NTPDases) dephosphorylate nucleoside tri- and diphosphates to the corresponding di- and monophosphates. In the present study we synthesized adenine and uracil nucleotide mimetics, in which the phosphate residues were replaced
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