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92846

Sigma-Aldrich

5-Carboxyfluorescein N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Empirical Formula (Hill Notation):
C25H15NO9
CAS Number:
92557-80-7
Molecular Weight:
473.39
Beilstein:
7865828
MDL number:
MFCD00077322
UNSPSC Code:
12352108
PubChem Substance ID:
57653496
NACRES:
NA.32

product line

BioReagent

Quality Level

100

Assay

≥90% (HPLC)

form

powder

fluorescence

λex 492 nm; λem 520 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O

InChI

1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)

InChI key

TWOKNYRPKCLMAR-UHFFFAOYSA-N

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers. Fluorescent probe for nucleotides; Reagent for fluorescent derivatization in capillary electrophoresis

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G L Igloi
Analytical biochemistry, 233(1), 124-129 (1996-01-01)
A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluorescein. The product of
Lau, S.K., et al.
Journal of Chromatography A, 809, 203-203 (1998)
L G Lee et al.
Nucleic acids research, 20(10), 2471-2483 (1992-05-25)
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments
Bamaprasad Dutta et al.
Scientific reports, 7(1), 5765-5765 (2017-07-20)
Atherosclerosis arises from leukocyte infiltration and thickening of the artery walls and constitutes a major component of vascular disease pathology, but the molecular events underpinning this process are not fully understood. Proteins containing an Asn-Gly-Arg (NGR) motif readily undergo deamidation
Brandon L Blakely et al.
Nature methods, 11(12), 1229-1232 (2014-10-13)
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to

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