88793
Atto 532 NHS ester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
Synonym(s):
Atto 532
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About This Item
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product line
BioReagent
Quality Level
Assay
≥90% (HPLC)
≥90% (degree of coupling)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
λ
in methanol: water (1:1) (with 0.1% perchloric acid)
UV absorption
λ: 532-538 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 532 NHS ester is a fluorescent dye related to the well-known laser dye, Rhodamine 6G. The fluorescence activity is excited efficiently at the 515-545nm range. A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd: YAG laser.
Application
Atto 532 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.
Features and Benefits
Characteristic features of the Atto 532 NHS ester are:
- Strong Absorption.
- High Fluorescence quantum yield.
- High Photostability.
- Excellent water solubility.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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