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45253

Sigma-Aldrich

Wright Stain solution

for microscopy

Synonym(s):

Eosin Methylene blue solution according to Wright, Wright’s eosin methylene blue

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About This Item

MDL number:
MFCD00005038
UNSPSC Code:
12171500
PubChem Substance ID:
329756625
NACRES:
NA.47

grade

for microscopy

Quality Level

100

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

impurities

methanol

density

0.80 g/mL at 20 °C

suitability

Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

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General description

Wright Stain is a modified Romanowsky stain commonly used for the cellular elements of blood. Wright′s stain is a mixture of eosin Y with polychromed (oxidized and partly demethylated) methylene blue and therefore termed polychrome stains. The hematological stain is intended for differentially staining peripheral blood and bone marrow films.

Application

Wright stain has been used to determine the number of white blood cell types (neutrophils, lymphocytes, monocytes, eosinophils, and basophils) per microliter of whole blood.

Other Notes

Blood stain

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1

Target Organs

Eyes

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

51.8 °F

Flash Point(C)

11 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K. Leybold et al.
Praxis-Laboratorium: Grundlagen der Laboratoriumsdiagnostik, 24-24 (1976)
Borrelia burgdorferi has minimal impact on the Lyme disease reservoir host Peromyscus leucopus.
Schwanz LE, et al.
Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), 117-124 (2011)
Bernadette FR Jacqueline HC et al.
Clinical Hematology, 3rd Ed. Henry Kimpton (2015)
Analysis and testing of biological stains--the Biological Stain Commission Procedures.
Penney D P, et al.
Biotechnic & Histochemistry (2002)
Young-Joo Jeong et al.
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