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27816

Sigma-Aldrich

Brilliant Blue R

250, for microscopy

Synonym(s):

Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
6104-59-2
Molecular Weight:
825.97
Colour Index Number:
42660
Beilstein:
5718025
EC Number:
228-060-5
MDL number:
MFCD00041762
UNSPSC Code:
12171500
PubChem Substance ID:
57648236
NACRES:
NA.26

grade

for microscopy

Quality Level

200

form

powder

technique(s)

microbe id | staining: suitable

λmax

589.2 nm in ethanol

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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Application

For detection of protein bands following electrophoresis.

Other Notes

Protein stain for gel electrophoresis; 5 to 10 times more sensitive than Naphthol Blue Black B and Bromophenol (as little as 0.5 μg/cm2 of protein could be detected in a gel matrix)

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Camilla Pilotti et al.
Investigative ophthalmology & visual science, 61(5), 19-19 (2020-05-15)
The 32W and 32Q variants of complement factor B (CFB) are associated with reduced risk of developing neovascular age-related macular degeneration (AMD) compared with the common 32R allele. The objective of this study was to determine if the most protective
Virginia Caponera et al.
Journal of economic entomology, 113(1), 348-352 (2019-10-08)
Damage from termite infestations is economically significant and control can be costly when requiring the widespread use of conventional insecticides. Erythritol, a polyalcohol sweetener that is safe for human consumption, causes increased mortality when ingested by some insects, indicating potential
Jie Zang et al.
Journal of experimental botany, 71(19), 5896-5910 (2020-07-21)
Iron (Fe) is an essential micronutrient and plays an irreplaceable role in plant growth and development. Although its uptake and translocation are important biological processes, little is known about the molecular mechanism of Fe translocation within seed. Here, we characterized
A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.
A Chrambach et al.
Analytical biochemistry, 20(1), 150-154 (1967-07-01)
S Fazekas de St Groth et al.
Biochimica et biophysica acta, 71, 377-391 (1963-05-14)
Two new procedures are described for the estimation of protein by direct photometry on electrophoretic strips. The protein complexes of procion brilliant blue RS and coomassie brilliant blue R250 are shown to follow Beer's law up to 50 and 20
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