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About This Item
UNSPSC Code:
41106514
Biological source:
human esophagus
Relevant disease(s):
cancer
Growth mode:
Adherent
Morphology:
Epithelial growing in islands
Receptors:
Not specified
Technique(s):
cell culture | mammalian: suitable
Product Name
SK-GT-4 cell line human, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human esophagus
Quality Segment
form
liquid
manufacturer/tradename
ECACC
packaging
tube of 5 μg 11012007-DNA-5UG, pkg of vial of cells 11012007-1VL
growth mode
Adherent
karyotype
Modal number 59 chromosomal aberration at 11p13-15.
morphology
Epithelial growing in islands
products
Expression of actin imtermediate filaments and growth factors TGF?1, TGF?2, TGF?3, TGF? and platelet-derived growth factor A (PDGFA). P53 mutation c.298C>T
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
Application
For complete product information, please see ECACC
Recommended culture medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Biochem/physiol Actions
Oesophagus adenocarcinoma, well-differentiated
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Preparation Note
RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cell population doubling time 39 hours.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections supplied by us, are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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