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04272

Sigma-Aldrich

di-Sodium hydrogen phosphate dihydrate

meets analytical specification of Ph. Eur., BP, 98.5-101% (calc. to the dried substance)

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate dihydrate, Disodium phosphate, Sodium phosphate dibasic dihydrate

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About This Item

Linear Formula:
Na2HPO4 · 2H2O
CAS Number:
Molecular Weight:
177.99
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

Agency

meets analytical specifications of BP
meets analytical specifications of Ph. Eur.

Quality Level

Assay

98.5-101% (calc. to the dried substance)

form

crystals

quality

meets analytical specification of Ph. Eur., BP

impurities

reducing matter, complies
residual solvents, complies
≤0.001% heavy metals (as Pb)
≤1% NaH2PO4 (calc. to dried subst.)

loss

19.5-21% loss on drying, 130 °C

pH

9.0-9.4 (20 °C, 1%)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

As: ≤2 mg/kg
Fe: ≤10 mg/kg

suitability

complies for appearance of solution

SMILES string

O.O.[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P.2H2O/c;;1-5(2,3)4;;/h;;(H3,1,2,3,4);2*1H2/q2*+1;;;/p-2

InChI key

KDQPSPMLNJTZAL-UHFFFAOYSA-L

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Application

Sodium phosphate dibasic dihydrate has been used for isolating DNA and RNA from bacterial samples of human gastrointestinal (GI) tract.

Biochem/physiol Actions

Sodium phosphate dibasic dihydrate is an important component of running buffer of denaturing gel electrophoresis.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
Erwin G Zoetendal et al.
Nature protocols, 1(2), 870-873 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that develops in time and space. The most widely used approaches to study microbial diversity and activity are all based on the analysis of nucleic acids, DNA, rRNA and mRNA.
Sohini Mukherjee et al.
Nature communications, 6, 8467-8467 (2015-10-13)
Creating an artificial functional mimic of the mitochondrial enzyme cytochrome c oxidase (CcO) has been a long-term goal of the scientific community as such a mimic will not only add to our fundamental understanding of how CcO works but may
Patrizia Iavicoli et al.
Journal of chromatography. A, 1422, 260-269 (2015-10-27)
Asymmetric Flow Field-Flow Fractionation (AF4) combined with multidetector analysis form a promising technique in the field of nanoparticle characterization. This system is able to measure the dimensions and physicochemical properties of nanoparticles with unprecedented accuracy and precision. Here, for the
Christopher Polonyi et al.
Dalton transactions (Cambridge, England : 2003), 43(31), 11941-11949 (2014-06-27)
The ability of the anticancer active drug cisplatin to exert biological activity through interference with nucleic acid function is well documented. Since kinetics play a key role in determining product distributions in these systems, methods for accurate documentation of reactivity

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