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Y1500

Sigma-Aldrich

YPD Agar

Synonym(s):

Yeast Extract Peptone Dextrose Agar, YEPD Agar

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About This Item

UNSPSC Code:
50405901
eCl@ss:
42040102
NACRES:
NA.75

form

powder

Quality Level

200

composition

Agar, 15 g/L
Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

application(s)

food and beverages

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Related Categories

General description

YPD agar is a complete yeast culture medium for most yeast species, especially for Saccharomyces cerevisiae. It is also known as YEPD and comprises of yeast extract, bacteriological peptone, glucose, and agar. The components of this media provide all the necessary nutrients for yeast growth. The essential vitamins, minerals and amino acids in the medium acts carbon and nitrogen source.

Application

YPD agar has been used as a culture media:
  • to cultivate S. cerevisiae strains to find a long-term preservation method for a deep space biosensor mission
  • for yeast strains in in vitro broth microdilution (BMD) based on minimum inhibitory concentration (MIC)
  • for Zymoseptori triciti strains for virulence studies

Preparation Note

1. Suspend 65 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000400585
0000357358
0000379714
0000338554
0000274275

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Ingrid A Edwards et al.
ACS infectious diseases, 3(12), 917-926 (2017-09-30)
Tachyplesin-1 (TP1; 1) is a cationic β-hairpin antimicrobial peptide with a membranolytic mechanism of action. While it possesses broad-spectrum, potent antimicrobial activity, 1 is highly hemolytic against mammalian erythrocytes, which precludes it from further development. In this study, we report
D A Treco et al.
Current protocols in molecular biology, Chapter 13, Unit13-Unit13 (2008-02-12)
Preparation of sterile media of consistently high quality is essential for the genetic manipulation of yeast. Recipes for media needed in the protocols in this chapter are provided in this unit. Specific suppliers are recommended for specific ingredients.
Xiao Liu et al.
Materials (Basel, Switzerland), 13(16) (2020-08-14)
Ammonia nitrogen and natural organic matter (NOM) seriously degrade the quality of surface waters. In this study, the optimum preparation conditions of a yeast-chitosan bio-microcapsule of the Candida tropicalis strain, used to treat micro-polluted surface water, were investigated. Fourier transform
Ilias Kounatidis et al.
G3 (Bethesda, Md.), 8(5), 1637-1647 (2018-03-15)
Candida glabrata (C. glabrata) forms part of the normal human gut microbiota but can cause life-threatening invasive infections in immune-compromised individuals. C. glabrata displays high resistance to common azole antifungals, which necessitates new treatments. In this investigation, we identified five
William T Kay et al.
Fungal genetics and biology : FG & B, 128, 14-19 (2019-03-17)
Zymoseptoria tritici, the causal agent of Septoria tritici blotch, is a notable pathogen of temperate-grown wheat. To better understand the mechanisms underpinning pathogenicity, leaf infection assays are commonly used to compare either the virulence of Z. tritici wildtype or mutant
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Protocols

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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