Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

U1250

Sigma-Aldrich

Urea

ReagentPlus®, ≥99.5%, pellets

Synonym(s):

Carbamide, Carbonyldiamide

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Linear Formula:
NH2CONH2
CAS Number:
57-13-6
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
200-315-5
MDL number:
MFCD00008022
UNSPSC Code:
12352100
PubChem Substance ID:
57654718
NACRES:
NA.21

Quality Level

300

product line

ReagentPlus®

Assay

≥99.5%

form

pellets

mp

132-135 °C (lit.)

solubility

H2O: 8 M

density

1.335 g/mL at 25 °C (lit.)

functional group

amine

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759) , CA2(760)
rat ... Ppm1a(24666)

Looking for similar products? Visit Product Comparison Guide

General description

Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. Urea can also disturb hydrogen bonds present in DNA secondary structure.

Application

Urea has been used as a protein denaturing agent.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000407853
0000399796
0000371712
0000371713
0000371715

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 4

1 of 4

Urea 8 M (after reconstitution with 16 mL high purity water)

Supelco

U4883

Urea

Urea BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

Sigma-Aldrich

U0631

Urea

Urea for biochemistry

Millipore

1.08488

Urea

Urea crystalline, EMPROVE® ESSENTIAL, Ph. Eur., BP, ChP, JP, USP

SAFC

1.08486

Urea

Sheehan D.
Physical Biochemistry: Principles and Applications (2013)
Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay.
Georgiou C D, et al.
Redox Biology, 17, 236-245 (2018)
Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2, 4-dinitrophenylhydrazine-based photometric assay.
Georgiou C D, et al.
Redox Biology, 17, 128-142 (2018)
Christos D Georgiou et al.
Redox biology, 17, 236-245 (2018-05-05)
A new fluorometric assay is presented for the ultrasensitive quantification of total protein carbonyls, and is based on their specific reaction with rhodamine B hydrazide (RBH), and the production of a protein carbonyl-RBH hydrazone the fluorescence of which (at ex/em
Florin Sasarman et al.
Human molecular genetics, 24(10), 2841-2847 (2015-02-06)
Addition of the trinucleotide cytosine/cytosine/adenine (CCA) to the 3' end of transfer RNAs (tRNAs) is essential for translation and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cytoplasm and mitochondria. Exome sequencing revealed TRNT1
View All Related Papers

Protocols

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service