E4382

Eosin Y disodium salt

Dye content, ≥90%, certified by the Biological Stain Commission, powder

• Synonym(s): 2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, D&C;Red No. 22, Eosin yellowish
• Empirical Formula (Hill Notation): C₂₀H₆Br₄Na₂O₅
• CAS Number: 17372-87-1
• Molecular Weight: 691.85
• Colour Index Number: 45380
• Beilstein: 3586809
• EC Number: 241-409-6
• MDL number: MFCD00005040
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894551
• NACRES: NA.47

Properties

• product name: Eosin Y disodium salt, certified by the Biological Stain Commission
• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, ≥90%
• technique(s): titration: suitable
• color: brown
• pH: 6.55 (29 °C, 84 °F)
• mp: 255 — 270 °C (491 - 518 °F at 977.9 hPa)
• solubility: H₂O: 1 mg/mL
• density: 1.018 g/cm³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
• InChI: 1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChI key: SEACYXSIPDVVMV-UHFFFAOYSA-L

Description

Application

Eosin Y disodium salt has been used as a counterstain in immunocytochemistry and immunohistochemistry.

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Suitability

Certified for use as a counterstain against hematoxylin; as a constituent of Wright stain; in modified Nocht′s stain; as a constituent in eosin-methylene blue medium.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H319
• Precautionary Statements: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): 376.9 °F - Pensky-Martens closed cup
• Flash Point(C): 191.6 °C - Pensky-Martens closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves