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410217

Sigma-Aldrich

2′,7′-Dichlorofluorescein

ACS reagent

Synonym(s):

Dichlorofluorescein; Fluorescein 27

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About This Item

Empirical Formula (Hill Notation):
C20H10Cl2O5
CAS Number:
76-54-0
Molecular Weight:
401.20
Beilstein:
58009
EC Number:
200-968-6
MDL number:
MFCD00005047
UNSPSC Code:
12171500
PubChem Substance ID:
24865700
NACRES:
NA.47

grade

ACS reagent

Quality Level

200

form

powder

technique(s)

titration: suitable

color

orange to red-brown, powder

mp

280 °C (dec.) (lit.)

solubility

alcohol: passes test

density

0.790 g/cm3

λmax

509 nm

suitability

passes test for adsorption indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

VFNKZQNIXUFLBC-UHFFFAOYSA-N

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General description

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe.

Application

2′,7′-Dichlorofluorescein has been used to measure ROS (reactive oxygen species) formation in cells. It has been used as a fluorogenic substrate to study the peroxidase activity of saliva samples and extracts obtained from submandibular glands.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL8813
BCCK3080
BCCH5169
BCCF7954
BCBG1058V

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Andréa G K Ferreira et al.
Journal of cellular biochemistry, 113(1), 174-183 (2011-09-02)
The present study investigated the effects of chronic hyperprolinemia on oxidative and metabolic status in liver and serum of rats. Wistar rats received daily subcutaneous injections of proline from their 6th to 28th day of life. Twelve hours after the
L Foucaud et al.
Toxicology in vitro : an international journal published in association with BIBRA, 24(6), 1512-1520 (2010-07-20)
The aim of this study was to investigate whether carbon black (CB) nanoparticles might induce toxicity to monocytic cells in vitro via an oxidative stress mechanism involving formation of the lipid peroxidation product 4-hydroxynonenal (4-HNE) and the subsequent role of
Sabry M Attia
Archives of toxicology, 86(5), 725-731 (2011-12-31)
Two topoisomerase II inhibitors, etoposide and merbarone, were tested for the induction of dominant lethal mutations in male mice. Etoposide was administered at a dosage of 30 or 60 mg/kg. Merbarone was administered at a dosage of 40 or 80
Michael Price et al.
Journal of biomedical optics, 15(5), 051605-051605 (2010-11-09)
Fluorescent probes are frequently employed for the detection of different reactive oxygen and nitrogen species formed during the irradiation of photosensitized cells and tissues. Investigators often interpret the results in terms of information provided with the different probes without examining
Olga N Pakhomova et al.
Archives of biochemistry and biophysics, 527(1), 55-64 (2012-08-23)
Nanosecond pulsed electric field (nsPEF) is a novel modality for permeabilization of membranous structures and intracellular delivery of xenobiotics. We hypothesized that oxidative effects of nsPEF could be a separate primary mechanism responsible for bioeffects. ROS production in cultured cells
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