Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

32750

Supelco

3,3′-Diaminobenzidine tetrahydrochloride hydrate

for spectrophotometric det. of Se, ≥97.5%

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Linear Formula:
C12H14N4 · 4HCl · xH2O
CAS Number:
868272-85-9
EC Number:
231-018-9
MDL number:
MFCD08273058
UNSPSC Code:
41116105
PubChem Substance ID:
329754314
NACRES:
NA.21

Quality Level

100

Assay

≥97.5%

quality

for spectrophotometric det. of Se

technique(s)

UV/Vis spectroscopy: suitable

SMILES string

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) is toxic in nature, and is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels.

Application

3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex.

Other Notes

Reagent for the spectrophotometric determination of selenium; Peroxidase stain. Differentiation between two classes of mycobacterial catalase in polyacrylamide electrophoresis gels; Specific indication of hemoproteins in polyacrylamide gels by double staining

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H319,H341,H350

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCV5872
MKCW7760
MKCK5098
BCCC2073
BCBV7283

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Interleukin-8 in the Human Endometrium 1.
Ariciri A
J. Clin. Endocr., 83 (5), 1783-1787 (1998)
Danzuka, T. and Ueno, K.
Analytical Chemistry, 30, 1370-1370 (1958)
L G Wayne et al.
Analytical biochemistry, 157(1), 89-92 (1986-08-15)
Mycobacteria produce two classes of catalase, designated T and M. Only the T-catalase also has a peroxidase-like function. When a 3,3'-diaminobenzidine (DAB) peroxidase stain was applied to polyacrylamide gel electrophoresis gels, followed by a ferricyanide negative stain for catalase, isoenzymes
HistoGreen: a new alternative to 3, 3'-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) as a peroxidase substrate in immunohistochemistry"
Thomas MA and Lemmer B
Brain Research, 14 (2), 107-118 (2005)
R T Francis et al.
Analytical biochemistry, 136(2), 509-514 (1984-02-01)
Hemoproteins were revealed in polyacrylamide gels in the presence of sodium dodecyl sulfate by staining with different benzidine derivatives. When the protein samples were treated with either beta-mercaptoethanol or dithiothreitol, a significant decrease in peroxidase activity of the proteins possessing
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service