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Key Documents

MAB5520

Sigma-Aldrich

Anti-CSPG4 Antibody

CHEMICON®, mouse monoclonal, 7.1

Synonym(s):

NG2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1, ascites fluid, clone 7.1, Chemicon®

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

7.1, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001897.4

UniProt accession no.

Q6UVK1

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CSPG4(1464)

Specificity

NG2 (chondroitin sulfate proteoglycan), human which is the homologue of the rat NG2 molecule. The antibody reacts with a 220-240 kD surface protein whose N-terminal sequence is identical to the rat NG2 chondrotin sulfate proteoglycan molecule.

MAB5520 reacts with only neoplastic leukocytes with specific association with chromosome 11q23 translocations involving the MLL gene. It has a very high specificity for t(4;11) or t(11;19) translocations. The antibody shows no reactivity with normal marrow hematopoietic cells, peripheral blld leukocytes, red blood cells or platelets. Leukemic cells lines KG1, KG1a, HRL, Jurkat, K562, Raji, HL-60, U937, Daudi, Nalm6 and CEM are negative. However MAB5520 does react with a variety of cell lines of nonhematopoietic origin, including HeLa cells.

Immunogen

Human bone marrow stromal cell line, SV40 transformed.

Application

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1 is an antibody against NG2 Chondroitin Sulfate Proteoglycan for use in FC & WB.
Western blot

Flow cytometry

Optimal working dilutions must be determined by end user.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Benign mesenchymal stromal cells in human sarcomas.
Morozov, A; Downey, RJ; Healey, J; Moreira, AL; Lou, E; Franceschino, A; Dogan, Y; Leung et al.
Clinical cancer research : an official journal of the American Association for Cancer Research null
Anne Therese Lauvrud et al.
Journal of tissue engineering and regenerative medicine, 11(9), 2490-2502 (2016-02-03)
Autologous fat grafting is a popular method for soft tissue reconstructions but graft survival remains highly unpredictable. Supplementation of the graft with the stromal vascular fraction (SVF) or cultured adipose tissue-derived stem cells (ASCs) can enhance graft viability. In this

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