AB9220
Anti-Nanog Antibody
serum, Chemicon®
Synonym(s):
Anti-NANOG/STM1, Anti-STM1
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
western blot: suitable
input
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NANOG(79923)
mouse ... Nanog(71950)
General description
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
Specificity
Recognizes Nanog, human.
Immunogen
Synthetic peptide from human Nanog.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.
Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.
Optimal working dilutions must be determined by the end user.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.
Optimal working dilutions must be determined by the end user.
Target description
39 kDa (MW 34 kDa)
Physical form
Rabbit Serum. Liquid.
Unpurified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Whole cell lysate from mouse embryonic stem cells
Whole cell lysate from mouse embryonic stem cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.
Hu, G; Kim, J; Xu, Q; Leng, Y; Orkin, SH; Elledge, SJ
Genes & Development null
Difei Dong et al.
Gene expression, 15(4), 153-162 (2012-07-13)
FoxA1 belongs to the fork head/winged-helix transcription factor family and participates in stimulating neuronal differentiation of pluripotent stem cells at early stages. To explore the biological roles of FoxA1 during this process, the stable expression of a GFP-FoxA1 fusion protein
Miguel Mendivil-Perez et al.
International journal of molecular sciences, 24(10) (2023-05-27)
Alzheimer's disease (AD) is a chronic neurological condition characterized by the severe loss of cholinergic neurons. Currently, the incomplete understanding of the loss of neurons has prevented curative treatments for familial AD (FAD). Therefore, modeling FAD in vitro is essential
Arlette A Haidar-Montes et al.
Animals : an open access journal from MDPI, 14(15) (2024-08-10)
Adipose-derived stem cells (ADSCs) hold promise for tendon repair, even if their tenogenic plasticity and underlying mechanisms remain only partially understood, particularly in cells derived from the ovine animal model. This study aimed to characterize oADSCs during in vitro expansion
Hee-Ryang Kim et al.
Journal of veterinary science, 18(3), 299-306 (2016-07-28)
Mesenchymal stem cells (MSCs) isolated from various tissues have been well characterized for therapeutic application to clinical diseases. However, in contrast to MSCs from other animal species, the characteristics of feline MSCs have not been fully documented. In this study
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service