AB2033
Anti-Fibronectin Antibody
CHEMICON®, rabbit polyclonal
Synonym(s):
Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
product name
Anti-Fibronectin Antibody, Chemicon®, from rabbit
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Fn1(14268)
General description
Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma. The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane-bound fibronectin receptors on cell surfaces. The immunogen for fibronectin polyclonal antibody AB2033 is purified mouse plasma. The antibody shows less than 0.1% reactivity with mouse laminin, and collagen types I, III, and IV by radioimmunoassay.
Specificity
This antibody reacts with mouse fibronectin. Shows less than 0.1% reactivity with mouse Laminin, and Collagen Types I, III, and IV by radioimmunoassay.
Immunogen
Fibronectin extracted and purified from mouse plasma.
Application
Detect Fibronectin using this Anti-Fibronectin Antibody validated for use in ELISA, IC, IH, RIA.
Immunohistochemistry:
1:80 dilution of a previous lot was used in immunofluorescent staining of frozen mouse skin and liver tissues.
Radioimmunoassay:
A previous lot of this antibody was used in RIA.
ELISA:
A 1:200 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
1:80 dilution of a previous lot was used in immunofluorescent staining of frozen mouse skin and liver tissues.
Radioimmunoassay:
A previous lot of this antibody was used in RIA.
ELISA:
A 1:200 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Quality
Evaluated by immunocytochemistry on NIH/3T3 cells.
Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of NIH/3T3 using AB2033 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue).
Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of NIH/3T3 using AB2033 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue).
Physical form
ImmunoAffinity Purified
The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Extracellular matrix in most tissue
Extracellular matrix in most tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Laetitia Sabatier et al.
The Biochemical journal, 456(2), 283-295 (2013-09-28)
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