07-577
Anti-Stargazin/Cacng2 Antibody, CT
Upstate®, from rabbit
Synonym(s):
Neuronal voltage-gated calcium channel gamma-2 subunit, calcium channel, voltage-dependent, gamma subunit 2, voltage-dependent calcium channel gamma-2 subunit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Cacng2(12300)
rat ... Cacng2(84347)
General description
Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state.
There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.
There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.
Specificity
Predicted to cross-react with mouse based on sequence similarity.
Recognizes Stargazin, MW ~36-39 kDa.
Immunogen
Epitope: C-terminus
KLH-conjugated, synthetic peptide corresponding to amino acids 305-323 (KDSKDSLHANTANRRTTPV) of rat Stargazin.
Application
Immunoprecipitation:
2-5 µg of this antibody has been reported by an independent laboratory to immunoprecipitate Stargazin in rat P2 lysate.
2-5 µg of this antibody has been reported by an independent laboratory to immunoprecipitate Stargazin in rat P2 lysate.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
This Anti-Stargazin/Cacng2 Antibody, CT is validated for use in IC, IP, WB for the detection of Stargazin/Cacng2.
Quality
Evaluated by western blot on RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).
Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected Stargazin in RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).
Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected Stargazin in RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).
Target description
~36-39 kDa
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Brain tissue
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Nonchannel functions of the calcium channel gamma subunit: insight from research on the stargazer mutant.
Qiao, Xiaoxi and Meng, Hongdi
Journal of Bioenergetics and Biomembranes, 35, 661-670 (2003)
L Yu et al.
Neuroscience, 178, 13-20 (2011-01-25)
Changes in AMPA receptors have been proposed to underlie changes in synaptic efficacy in hippocampus and other brain structures. Calpain activation has also been discussed as a potential mechanism to produce lasting modifications of synaptic structure and function. Stargazin is
Maureen G Price et al.
The Journal of biological chemistry, 280(20), 19711-19720 (2005-03-12)
Mutations in the Cacng2 gene encoding the neuronal transmembrane protein stargazin result in recessively inherited epilepsy and ataxia in "stargazer" mice. Functional studies suggest a dual role for stargazin, both as a modulatory gamma subunit for voltage-dependent calcium channels and
New transmembrane AMPA receptor regulatory protein isoform, gamma-7, differentially regulates AMPA receptors.
Kato, AS; Zhou, W; Milstein, AD; Knierman, MD; Siuda, ER; Dotzlaf, JE; Yu, H; Hale et al.
The Journal of Neuroscience null
Akihiko S Kato et al.
Nature medicine, 22(12), 1496-1501 (2016-11-08)
Pharmacological manipulation of specific neural circuits to optimize therapeutic index is an unrealized goal in neurology and psychiatry. AMPA receptors are important for excitatory synaptic transmission, and their antagonists are antiepileptic. Although efficacious, AMPA-receptor antagonists, including perampanel (Fycompa), the only
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