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906603

Sigma-Aldrich

DLAM-Iδ1Tγ-13CH3 Methyl Labeling Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.12

technique(s)

bio NMR: suitable

Quality Level

200

shipped in

dry ice

storage temp.

−70°C

General description

DLAM-Iδ1Tg-13CH3 kit has 13CH3isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.

Application

DLAM-Iδ1Tg-13CH3 is an optimized kit to produce proteins with 13CH3 isotopomer on the methyl groups of isoleucine and threonine. This kit has been tested with protein isotopic labeling in E. coli. It is used in methyl-TROSY NMR for the investigation of the active site of the proteasome and also in the study of catalytic mechanism.
For detection of long-range nOes or for the NMR study of large proteins

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

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Algirdas Velyvis et al.
Journal of the American Chemical Society, 135(25), 9259-9262 (2013-06-27)
The 20S proteasome core particle is a molecular machine that plays a central role in the regulation of cellular function through proteolysis, and it has emerged as a valuable drug target for certain classes of cancers. Central to the development
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

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