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T8453

Sigma-Aldrich

Anti-β-Tubulin II antibody, Mouse monoclonal

clone 7B9, purified from hybridoma cell culture

Synonym(s):

Anti-TUBB2, Anti-Tubulin, β2

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

7B9, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human, mouse, rat

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Related Categories

General description

Monoclonal Anti- β-Tubulin II (mouse IgG1 isotype) is derived from the hybridoma 7B9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment at the C-terminus of human β?tubulin II. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer that consists of α-tubulin and β ?tubulin. Both subunits have a molecular mass of ~50 kDa and share considerable homology.

Application

Anti-β-Tubulin II antibody has been used in
  • immunoblotting immunoprecipitation
  • immunocytochemistry
  • western blotting
  • immunolabelling

Biochem/physiol Actions

The αβ tubulins are involved in the formation of microtubules during cell division by the formation of protofilaments.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hongfeng Yuan et al.
Oncotarget, 8(9), 14912-14924 (2017-01-25)
Diffuse large B-cell lymphoma (DLBCL) is one of the most aggressive non-Hodgkin lymphomas. It is curable but one-third of cases are refractory to therapy or relapse after initial response highlighting the urgent need for developing novel therapeutic approaches. Targeting sirtuins
Mutations affecting beta-tubulin folding and degradation
Wang Y, et al.
The Journal of Biological Chemistry, 281(19), 13628-13635 (2006)
Spatio-temporal dynamics of $\beta$-tubulin isotypes during the development of the sensory auditory organ in rat
Renauld J, et al.
Histochemistry and Cell Biology, 144(5), 403-416 (2015)
Salama A Salama et al.
Fertility and sterility, 98(1), 178-184 (2012-05-15)
To investigate the effects and the mechanism of action of 2-methoxyestradiol (2ME(2)) on transforming growth factor (TGF) β3-induced profibrotic response in immortalized human uterine fibroid smooth muscle (huLM) cells. Laboratory study. University research laboratory. Not applicable. Not applicable. huLM cells
FOXP1 potentiates Wnt/ beta-catenin signaling in diffuse large B cell lymphoma
Walker M P, et al.
Science Signaling, 8(362), ra12-ra12 (2015)

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