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S4944

Sigma-Aldrich

Monoclonal Anti-Sonic Hedgehog (N-terminal) antibody produced in rat

clone 171018, purified immunoglobulin, lyophilized powder

Synonym(s):

Anti-Shh

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About This Item

MDL number:
MFCD06798717
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

rat

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

171018, monoclonal

form

lyophilized powder

species reactivity

human, mouse

technique(s)

capture ELISA: suitable
immunohistochemistry (frozen sections): 25 μg/mL using mouse embryo
neutralization: suitable
western blot: 1-2 μg/test

isotype

IgG2a

UniProt accession no.

Q15465

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SHH(6469)
mouse ... Shh(20423)

Immunogen

purified, E. coli-derived, recombinant mouse sonic hedgehog N-terminal peptide (amino acids 25-198).

Biochem/physiol Actions

Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, gamma catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Raffaella Bonavita et al.
Biology open, 7(7) (2018-05-23)
In humans, the sacrum forms an important component of the pelvic arch, and it transfers the weight of the body to the lower limbs. The sacrum is formed by collapse of the intervertebral discs (IVDs) between the five sacral vertebrae
Chitra Lekha Dahia et al.
PloS one, 7(4), e35944-e35944 (2012-05-05)
Intervertebral discs (IVD) are essential components of the vertebral column. They maintain separation, and provide shock absorbing buffers, between adjacent vertebrae, while also allowing movements between them. Each IVD consists of a central semi-liquid nucleus pulposus (NP) surrounded by a
Alessia Omenetti et al.
American journal of physiology. Gastrointestinal and liver physiology, 294(3), G595-G598 (2008-01-26)
Hedgehog (Hh) signaling modulates tissue remodeling by controlling the fate of Hh-responsive cells. Healthy adult livers exhibit little Hh activity. However, cells involved in adult liver repair, including myofibroblasts and progenitors, are capable of producing and responding to Hh ligands.
Fergal C Kelleher
Carcinogenesis, 32(4), 445-451 (2010-12-28)
To conduct a systematic review of the role that the hedgehog signaling pathway has in pancreatic cancer tumorigenesis. PubMed search (2000-2010) and literature based references. Firstly, in 2009 a genetic analysis of pancreatic cancers found that a core set of
Shohreh F Farzan et al.
American journal of physiology. Gastrointestinal and liver physiology, 294(4), G844-G849 (2008-02-02)
The Hedgehog (Hh) family of secreted proteins is necessary for aspects of the development and maintenance of the gastrointestinal tract. Hh is thought to function as a morphogen, a mitogen, a cell survival factor, and an axon guidance factor. Given

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