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N1752

Sigma-Aldrich

4-Nitrophenyl β-D-lactopyranoside

powder

Synonym(s):

4-Nitrophenyl beta-D-lactopyranoside, p-Nitrophenyl-beta-D-lactopyranoside

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About This Item

Empirical Formula (Hill Notation):
C18H25NO13
CAS Number:
4419-94-7
Molecular Weight:
463.39
MDL number:
MFCD00069843
UNSPSC Code:
12352204
PubChem Substance ID:
24897521
NACRES:
NA.28

Assay

≥99% (TLC)

Quality Level

200

form

powder

color

white

solubility

water: 19.60-20.40 mg/mL, clear to slightly hazy, colorless to yellow

storage temp.

−20°C

SMILES string

OCC1OC(OC2C(O)C(O)C(OC2CO)Oc3ccc(cc3)N(=O)=O)C(O)C(O)C1O

InChI

1S/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2

InChI key

IAYJZWFYUSNIPN-UHFFFAOYSA-N

Application

4-Nitrophenyl β-D-lactopyranoside may be used as a substrate for determining β-lactosidase activity and for determining the substrate specificity for β-glucosidase from the yeast Debaryomyces hansenii. It has also been used as a substrate for cellobiohydrolase.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H360D

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mechanism of initial rapid rate retardation in cellobiohydrolase catalyzed cellulose hydrolysis
Jalak J and Valjamae P
Biotechnology and Bioengineering, 106(6), 871-883 (2010)
Extraction and immobilization in one step of two beta-glucosidases released from a yeast strain of Debaryomyces hansenii
Riccio P, et al.
Enzyme and Microbial Technology, 24(3-4), 123-129 (1999)
Nanna Røjel et al.
Biotechnology and bioengineering, 117(2), 382-391 (2019-10-22)
Understanding the pH effect of cellulolytic enzymes is of great technological importance. In this study, we have examined the influence of pH on activity and stability for central cellulases (Cel7A, Cel7B, Cel6A from Trichoderma reesei, and Cel7A from Rasamsonia emersonii).
Keyong Li et al.
Cell reports, 34(5), 108714-108714 (2021-02-04)
Brainstem networks that control regular tidal breathing depend on excitatory drive, including from tonically active, CO2/H+-sensitive neurons of the retrotrapezoid nucleus (RTN). Here, we examine intrinsic ionic mechanisms underlying the metronomic firing activity characteristic of RTN neurons. In mouse brainstem

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