FLAAM

Adenosine 5′-triphosphate (ATP) assay mix

lyophilized powder

• Synonym(s): ATP assay kit, ATP detection kit
• NACRES: NA.84
• eCl@ss: 32160414
• UNSPSC Code: 41116133

Properties

• form: lyophilized powder
• Quality Segment: 200
• usage: vial sufficient for ≥50 assays
• technique(s): PCR: suitable, protein detection: suitable
• storage temp.: −20°C

Description

General description

The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin.

Application

The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes.

Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay.

Preparation Note

Reconstitute with 5 ml of sterile water.

Other Notes

Contains luciferase, luciferin, MgSO₄, EDTA, DTT, BSA, and Tricine buffer.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Codes: H318
• Precautionary Statements: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)