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A0481

Sigma-Aldrich

Anti-Axin1 (C-terminal) antibody produced in rabbit

enhanced validation

1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Axin, Anti-Axis Inhibitor 1, Anti-Axis inhibition protein 1

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About This Item

MDL number:
MFCD02095870
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen ~130 kDa

species reactivity

rat, mouse

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

1.5 mg/mL

technique(s)

western blot: 0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1.

UniProt accession no.

O35625

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AXIN1(8312)
mouse ... Axin1(12005)
rat ... Axin1(79257)

General description

Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC),,,.
Rabbit Anti-Axin1 (C-terminal) antibody is specific for rat and mouse axin1 by immunoblotting (∼130 kDa). Staining of the axin1 band in immunoblotting is specifically inhibited by the immunizing peptide

Immunogen

synthetic peptide corresponding to amino acids 815-832 located near the C-terminal of rat axin1, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in human, mouse, and bovine axin1.

Application

Anti-Axin1 (C-terminal) antibody is suitable for use in western blot (0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1). The antibody has also been used for immunoblotting assays in astrocytoma cell lysates.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lobna Elkhadragy et al.
Frontiers in oncology, 12, 904031-904031 (2022-06-08)
Hepatocellular carcinoma (HCC) is an aggressive disease lacking effective treatment. Animal models of HCC are necessary for preclinical evaluation of the safety and efficacy of novel therapeutics. Large animal models of HCC allow testing image-guided locoregional therapies, which are widely
L Zeng et al.
Cell, 90(1), 181-192 (1997-07-11)
Mutations at the mouse Fused locus have pleiotropic developmental effects, including the formation of axial duplications in homozygous embryos. The product of the Fused locus, Axin, displays similarities to RGS (Regulators of G-Protein Signaling) and Dishevelled proteins. Mutant Fused alleles
F Fagotto et al.
The Journal of cell biology, 145(4), 741-756 (1999-05-20)
Axin was identified as a regulator of embryonic axis induction in vertebrates that inhibits the Wnt signal transduction pathway. Epistasis experiments in frog embryos indicated that Axin functioned downstream of glycogen synthase kinase 3beta (GSK3beta) and upstream of beta-catenin, and
Li-Ying Zhang et al.
International journal of oncology, 35(1), 25-32 (2009-06-11)
Astrocytic tumors are the most common brain tumors with various genetic defects. As a tumor suppressor gene, Axin could control cell death and growth. Axin possesses a separate domain that directly interacts with p53 and regulates the activity of p53
H Yamamoto et al.
Molecular and cellular biology, 18(5), 2867-2875 (1998-05-05)
Using a yeast two-hybrid method, we identified a novel protein which interacts with glycogen synthase kinase 3beta (GSK-3beta). This protein had 44% amino acid identity with Axin, a negative regulator of the Wnt signaling pathway. We designated this protein Axil
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