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Trizma® hydrochloride buffer solution

BioUltra, for molecular biology, pH 7.4

Synonym(s):

Tris(hydroxymethyl)aminomethane hydrochloride buffer solution

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About This Item

Beilstein:
3675235
MDL number:
MFCD00012590
UNSPSC Code:
12000000
eCl@ss:
32129211
PubChem Substance ID:
329770223
NACRES:
ND.02

grade

for molecular biology

Quality Level

100

product line

BioUltra

form

liquid

concentration

1.0 M tris-HCl

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

7.4±0.1 (25 °C)

density

1.05 g/mL at 20 °C

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.04

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Application


  • Reduced glutathione inhibits rabbit and rat skeletal muscle lactate dehydrogenase and prevents dinitrophenol induced extracellular acidification by an epithelial cell line.: This study investigates how Trizma® hydrochloride buffer solution maintains pH stability, critical for accurately assessing the biochemical interactions in muscle and epithelial cells (Wimberger et al., 1997).

Other Notes

Buffer component (SET buffer) in the preparation of RNA

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL3799
BCCL9105
BCCK6562
BCCK1058
BCCK3909

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Sigma-Aldrich

T2944

Trizma® hydrochloride solution

Isolation of RNA.
S L Berger et al.
Methods in enzymology, 180, 3-13 (1989-01-01)
Anna Iwaniak et al.
International journal of molecular sciences, 22(6) (2021-04-04)
In silico and in vitro methods were used to analyze ACE- and DPP-IV-inhibiting potential of Gouda cheese with a modified content of β-casein. Firstly, the BIOPEP-UWM database was used to predict the presence of ACE and DPP-IV inhibitors in casein
Satish N Dighe et al.
ACS omega, 5(48), 30971-30979 (2020-12-17)
Cholinesterases (ChE) are well-known drug targets for the treatment of Alzheimer's disease (AD). In continuation of work to develop novel cholinesterase inhibitors, we utilized a structure-based scaffold repurposing approach and discovered six novel ChE inhibitors from our recently developed DNA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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