AGRLE-RO

Agarose LE

low electroendosmosis

• Synonym(s): agarose
• UNSPSC Code: 12352200

Properties

• biological source: algae (seaweed)
• form: powder
• packaging: pkg of 100 g (11685660001); pkg of 500 g (11685678001)
• manufacturer/tradename: Roche
• EEO: 0.05 -0.13
• transition temp: gel point 36 °C ±1.5
• gel strength: >=2,500 g/cm²
• foreign activity: Dnase, none detected; Rnase, none detected
• shipped in: ambient
• storage temp.: 20-25°C

Description

General description

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

Application

Agarose LE has been used for:

• analyzing fragments between 0.2 and 15 kb
• analysis of PCR products
• examination of restriction endonucleases
• digests of plasmid, cosmid, and λ phage DNA
• electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
• in combination with ethidium bromide ​in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.

Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

Quality

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash