11767305001
Roche
TUNEL Enzyme
from calf thymus recombinant in E. coli
Synonym(s):
transferase dUTP nick end labeling, tunel
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About This Item
UNSPSC Code:
41105600
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
solution
usage
sufficient for 20 tests
packaging
pkg of 2 × 50 μL
manufacturer/tradename
Roche
storage temp.
−20°C
General description
TUNEL enzyme solution, containing terminal deoxynucleotidyl transferase from calf thymus recombinant in E. coli.
One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.
One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.
Application
The enzyme solution contains terminal deoxynucleotidyl transferase from calf thymus, recombinant in E. coli, needed to perform the TUNEL reaction for detecting apoptosis in situ.
The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.
The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.
Physical form
Terminal deoxynucleotidyl transferase (TdT) in an optimized concentration, dissolved in reaction buffer. Ready-to-use with TUNEL Label.
Preparation Note
Working solution: TUNEL Enzyme is used in combination with TUNEL Label to prepare the TUNEL reaction mixture.
To produce a TUNEL solution sufficient for one test:
Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.
To produce a TUNEL solution sufficient for one test:
- Mix 5μl TUNEL Enzyme with 45μl TUNEL Label prior to use.
- For negative control use 50μl/test TUNEL Label only.
Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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