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Tetramethyl-Rhodamine-5-dUTP

Synonym(s):

Rhodamine-5-dUTP tetralithium salt, Tetramethylrhodamine-5(6)-(5-[3-carboxamidoallyl]-2′-deoxyuridine 5′-triphosphate)

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About This Item

Empirical Formula (Hill Notation):
C37H40N5O18P3
Molecular Weight:
935.66
UNSPSC Code:
41116100

grade

Molecular Biology

Quality Level

100

Assay

97.7% (HPLC)

form

solution

mol wt

990.5

packaging

pkg of 25 μL (25 nmol; 1mM)

manufacturer/tradename

Roche

concentration

1.0 mmol/L (Tetramethylrhodamine-5-dUTP (Abs. 551 nm))

color

clear red

mp

~0 °C

solubility

water: miscible

storage temp.

−20°C

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General description

Tetramethylrhodamine is bound to deoxyuridine triphosphate via an amide linkage.

Application

Tetramethyl-Rhodamine-5-dUTP has been used in the labelling of DNA fragments and in the synthesis of fluorescence in situ hybridization (FISH) probes.

Quality

Typical analysis: At least 85% Tetramethyl-Rhodamine-5-dUTP (HPLC, area%).

Analysis Note

Absorption: Absorption spectra available in the files.

Other Notes

For life science research only. Not for use in diagnostic procedures. For some in situ experiments, it is useful to label DNA with two fluorescent "tags," tetramethylrhodamine (red) and fluorescein (yellow).

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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