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810130P

Avanti

16:0-06:0 NBD PC

Avanti Research - A Croda Brand 810130P, powder

Synonym(s):

1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine

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About This Item

Empirical Formula (Hill Notation):
C36H62N5O11P
CAS Number:
Molecular Weight:
771.88
UNSPSC Code:
12352211
NACRES:
NA.25

Assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 mg (810130P-1mg)
pkg of 5 × 1 mg (810130P-5mg)

manufacturer/tradename

Avanti Research - A Croda Brand 810130P

shipped in

dry ice

storage temp.

−20°C

General description

16:0-06:0 NBD PC (1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine) is a fluorescent analogue of phosphatidylcholine.

Application

16:0-06:0 NBD PC (1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine) has been used in NBD-phospholipid translocation assay. It may be used as a molecular fluorescent marker to label lipids during the friction tests, as substrate for enzyme substrate specificity determination and in the preparation of small unilamellar vesicles.

Biochem/physiol Actions

16:0-06:0 NBD PC (1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine) acts as a potential fluorescent probe. It is used for the quantification of the soluble and membrane-bound phospholipase A2 (PLA2) activity. 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) derivatives are widely used as fluorescent analogs of native lipids in biological and model membranes to study a variety of processes.

Packaging

5 mL Amber Glass Screw Cap Vial (810130P-1mg)
5 mL Amber Glass Screw Cap Vial (810130P-5mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Storage Class Code

11 - Combustible Solids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In vitro analysis of the Staphylococcus aureus lipoteichoic acid synthase enzyme using fluorescently labeled lipids
Karatsa-Dodgson MW, et al.
Journal of Bacteriology, 192(20), 5341-5349 (2010)
Effects of fluorescent probe NBD-PC on the structure, dynamics and phase transition of DPPC. A molecular dynamics and differential scanning calorimetry study
Loura L, et al.
Biochimica et Biophysica Acta - Biomembranes, 1778(2), 491-501 (2008)
Role of the biomolecular interactions in the structure and tribological properties of synovial fluid
Mirea DA, et al.
Tribology International, 59(3), 302-311 (2013)
The use of C6-NBD-PC for assaying phospholipase A2-activity: scope and limitations
Meyuhas D, et al.
Biochimica et Biophysica Acta, Lipids and Lipid Metabolism, 1124(3), 223-232 (1992)
Measuring translocation of fluorescent lipid derivatives across yeast Golgi membranes
Natarajan P, et al.
Methods, 39(2), 163-168 (2006)

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