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G005815

GC Analysis of Organophosphorus Pesticides (OP-Pest) in Cabbage, Onion, and Mushrooms on SLB-5ms after SPE using Supelclean™ ENVI-Carb™ II/PSA

application for SPE, suitable for GC

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About This Item

technique(s)

gas chromatography (GC): suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: 10 g homogenized food sample (green onions, apples, Napa cabbage, button mushrooms)
SPE tube/cartridge: Supelclean ENVI-Carb II/PSA SPE Tube (54067-U)
condition: 5 mL acetone:toluene (65:35)
sample addition: 1 mL sample extract
elution: 10 mL of acetone:toluene (65:35)
eluate post-treatment: Evaporate fraction to 0.5 mL then adjust volume to 1 mL with ethyl acetate for GC-NPD analysis
column: SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
oven: 110 °C (2 min), 2.5 °C/min to 205 °C, 10 °C/min to 310 °C (10 min)
inj. temp.: 250 °C
detector: NPD, 310 °C
carrier gas: helium, 0.9 mL/min constant flow
injection: 2 μL, splitless
liner: 4 mm I.D. dual tapered

suitability

application for SPE

application(s)

agriculture
food and beverages

Preparation Note

Sample pretreatment: To a 10 g sample of homogenized food (green onions, apples, Napa cabbage, button mushrooms) was added 10 mL acetonitrile and 4 g magnesium sulfate (that had been heated at 550 °C) and 1 g sodium chloride. After centrifugation, the supernatant was removed and mixed with 1 g magnesium sulfate and centrifuged. 5 mL of the supernatant was removed and evaporated to 1 mL for SPE loading.

Analysis Note

Organophosphorous pesticide use in agriculture is widespread due to the fact that they are more amenable to environmental degradation in comparison to organochlorine or organonitrogen compounds. There are a large number of pesticides in the organophosphorous group, and because of their potential health effects, they are of particular concern on produce imported from areas in which they are commonly used. This chromatogram illustrates the separation of 63 of these pesticides by GC on the SLB-5ms using nitrogen phosphorus detection (NPD).

Legal Information

ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC
Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Methamidophos
    Dichlorvos
    Acephate
    Omethoate
    Ethoprophos
    Dicrotophos
    Cadusafos
    Monocrotophos
    Phorate
    Terbufos
    Propetamphos
    Disulfoton
    Chloropyrifos-methyl
    Parathion-methyl
    O,O-Diethyl O-(4-nitrophenyl) phosphate
    Malathion
    Fenthion
    Parathion
    Isocarbophos
    Trichloronate
    Methidathion
    Fensulfothion
    Ethion
    Famphur
    Pyridaphenthion
    Phosmet
    Phosalone
    Azinphos-methyl
    Azinphos-ethyl
    Coumaphos
    Temephos
    Diazinon
    Dichlofenthion
    Dimethoate
    Phosfolan
    Fenitrothion
    Phenthoate
    See All (37)

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