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G004541

HPLC Analysis of Folinic Acid Enantiomers on Astec® CHIROBIOTIC® T

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] methanol; [B] acetic acid; [C] triethylamine; (100:1:0.5, A:B:C)
flow rate: 2 mL/min
column temp.: 25 °C
detector: UV, 230 nm
injection: 2 μL
sample: 5 mg/mL in methanol

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Folinic acid calcium salt hydrate, BioXtra, ≥99.0% (HPLC)

analytical column

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standard

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