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Discovery® HS C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

Discovery RP18 HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L1

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

20% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

300 m2/g

surface coverage

3.2 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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Application

Discovery® HS C18 HPLC column may be used for the separation of cefdinir and cefixime in plasma samples, followed by their analysis and detection using reversed-phase high performance liquid chromatography coupled with ultraviolet detector.

Features and Benefits

  • Stable, low bleed for LC-MS applications
  • Scalable from analytical to preparatory
  • Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility
  • Higher hydrophobicity for better resolution of difficult analytes
   

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

guard cartridge

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
291168-06
291168-05
291168-04
291168-03
291168-02

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Simultaneous determination of cefdinir and cefixime in human plasma by RP-HPLC/UV detection method: Method development, optimization, validation, and its application to a pharmacokinetic study.
Khan A, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 879(24), 2423-2429 (2011)
Jolanta Flieger et al.
Journal of separation science, 34(7), 733-739 (2011-03-02)
Highly hydrophilic compounds belonging to biogenic amines were analysed in the reversed-phase system, modified with the addition of ionic liquids: 1-ethyl-3-methyl-imidazolium hexafluorophosphate (EMIM PF(6)) and chaotropic salt NaPF(6) on Discovery HS C18 column at acidic conditions. The effect of the
Swati Jaiswal et al.
Journal of chromatographic science, 55(6), 617-624 (2017-03-24)
Tuberculosis (TB) with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome represents the most common infectious diseases worldwide. Anti-TB drugs are used concurrently with antiretroviral drug for treatment of TB-HIV co-morbidities. Due to lower risk of interaction with protease inhibitors, rifabutin is
Bhavin N Patel et al.
Biomedical chromatography : BMC, 22(11), 1213-1224 (2008-07-25)
A simple, precise and accurate assay for the determination of 6-methoxy-2-naphthylacetic acid (6-MNA), an active metabolite of nabumetone in human plasma, was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analyte (6-MNA) and propranolol (internal standard, IS) were
Fazli Khuda et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 944, 114-122 (2013-12-10)
A simple, specific, precise and rapid RP-HPLC-UV method was developed for simultaneous determination of lumefantrine and its metabolite desbutyl lumefantrine in human plasma. Experimental parameters were optimized and the method was validated according to standard guidelines. The method showed adequate
View All Related Papers

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HPLC Discovery HS C18

HPLC DiscoveryBIO HS C18 products. Available in 3µm, 5µm and 10µm particle sizes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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