V8012

Anti-V5 antibody, Mouse monoclonal

clone V5-10, purified from hybridoma cell culture

• Synonym(s): V5 Tag Monoclonal Antibody, V5 antibody
• NACRES: NA.56
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: V5-10, monoclonal
• Application: ICC, WB
• Citations: 144

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: V5-10, monoclonal
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide conjugated to KLH.

The mouse monoclonal Anti-V5 antibody reacts specifically with the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.

The mouse mooclonal Anti-V5 antibody reacts specifically with the V5-taged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable