T3195

Anti-γ-Tubulin (DQ-19) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-CDCBM4, Anti-GCP-1, Anti-TUBG, Anti-TUBGCP1
• MDL number: MFCD00677366
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 48 kDa
• species reactivity: chicken, human
• technique(s): indirect immunofluorescence: 1:500 using methanol-acetone fixed, cultured chicken fibroblasts.; western blot: 1:1,000 using whole cell extract of human epidermal carcinoma A431 cell line.
• UniProt accession no.: P23258
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBG1(7283) , TUBG2(27175)

Description

General description

Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells. It is a member of the tubulin superfamily of proteins which includes α- and β- tubulin and the newly discovered centrosomal-associated proteins, δ- and ε- tubulin. γ-Tubulin is a part of large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule. Its abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares 28-32% identity with α-tubulin from various organisms, 32-36% identity with β-tubulins and 29-30% identity with δ- and ε tubulins. Some regions including those thought to be involved in GTP) binding are highly conserved among α-, β-, γ-, δ-, and ε-tubulins.

Immunogen

synthetic peptide corresponding to the C-terminus of human γ-tubulin (amino acids 433-451 with N-terminally added lysine), conjugated to KLH.

Application

Anti-γ-Tubulin (DQ-19) antibody produced in rabbit has been used in:

• western blotting
• immunocytochemistry
• immunofluorescence

Biochem/physiol Actions

The proper organization of microtubules is essential for cell division and chromosome segregation, directed cell movement, interphase cytoplasmic organization, and other cytoskeletal functions. γ-Tubulin has an essential role in microtubule nucleation by the centrosomes. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-TuRC (γ-tubulin ring complex) binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable