SRP0699

STK9 Protein

recombinant

• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~84 kDa
• concentration: 0.1 μg/μL
• accession no.: NM_003159
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... STK9(6792)

Description

Application

Useful for Western blot.

Biochem/physiol Actions

Recombinant human STK9 (CDKL5) (1-498) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. STK9 or CDKL5 is a member of Ser/Thr protein kinase family which has protein kinase activity and is associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). STK9 can control nuclear speckle morphology by regulating the phosphorylation state of splicing regulatory proteins, and may be involved indirectly in pre-mRNA processing, by controlling splicing factor dynamics ⁽¹⁾. The mutations in the STK9 is a major cause of infantile spasms and early epileptic seizures in female patients, and of a later intractable seizure disorder ⁽²⁾.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable