SRP0280
UbcH13 (UBE2N) Active human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym(s):
UBC13 homolog, UBE2N, UbcH13, Ubiquitin-conjugating enzyme E2N
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in E. coli
Assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
17.6 kDa
packaging
pkg of 100 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... UBE2N(7334)
General description
UbcH13 (GenBank Accession No. NM_003348), amino acids 2-end, with N-terminal His-tag, MW = 17.6 kDa, expressed in an E. coli cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Certificates of Analysis (COA)
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