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COO/COA

SAB1409477

Anti-TRMT12 antibody produced in mouse

Name: Anti-TRMT12 antibody produced in mouse
Brand: SIGMA

purified immunoglobulin, buffered aqueous solution

Synonym(s):

FLJ20772, TRM12, TYW2

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

conjugate:

unconjugated

application:

WB

clone:

polyclonal

species reactivity:

human

citations:

2

technique(s):

western blot: 1 μg/mL

Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50.2 kDa

species reactivity

human

technique(s)

western blot: 1 μg/mL

NCBI accession no.

BC011713.2

UniProt accession no.

Q53H54

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TRMT12(55039)

General description

Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM

Immunogen

TRMT12 (AAH11713.1, 1 a.a. ~ 448 a.a) full-length human protein.

Sequence
MRENVVVSNMERESGKPVAVVAVVTEPRFTQRYREYLQRQKLFDTQHRVEKMPDGSVALPVLGETLPEQHLQELRNRVAPGSPCMLTQLPDPVPSKRAQGCSPAQKLCLEVSRWVEGRGVKWSAELEADLPRSWQRHGNLLLLSEDCFQAKQWKNLGPELWETVALALGVQRLAKRGRVSPDGTRTPAVTLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVDLYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIHFGDNRKLKLSNIADRVILGLIPSSEEGWPIACQVLRQDAGGILHIHQNVESFPGKNLQALGVSKVEKEHWLYPQQITTNQWKNGATRDSRGKMLSPATKPEWQRWAESAETRIATLLQQVHGKPWKTQILHIQPVKSYAPHVDHIVLDLECCPCPSVG

Biochem/physiol Actions

TRMT12 (tRNA methyltransferase 12 homolog) gene is amplified and over-expressed in breast cancer. This enzyme is responsible for catalyzing the third step in wybutosine biosynthetic pathway. In cancer, up-regulation of this gene leads to disruption in SMG1 protein function, which is a part of nonsense mediated decay pathway (NMD). Thus, it might contribute to the escape of damaged DNA from checkpoints.

Physical form

Solution in phosphate buffered saline, pH 7.4

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Lot/Batch Number

10344

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Chromosome 8 BAC array comparative genomic hybridization and expression analysis identify amplification and overexpression of TRMT12 in breast cancer.

Rodriguez V et al

Genes Chromosomes Cancer, 46(7), 694-707 (2007)

TRMT12 overexpression in cancer cells affects the cell cycle and the nonsense mediated decay pathways by regulating the SMG-1 kinase, a member of the PIKK family of kinases.