P4649

Phosphorylase b from rabbit muscle

For use as a marker in SDS-PAGE

• Synonym(s): α-Glucan Phosphorylase, 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase, Glycogen Phosphorylase
• CAS Number: 9012-69-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-737-0
• MDL number: MFCD00131905
• EC Number: 2.4.1.1 (BRENDA, IUBMB)

Properties

• Quality Segment: 200
• form: powder
• mol wt: observed mol wt ~97 kDa
• packaging: vial of 0.5 mg
• storage temp.: 2-8°C

Description

General description

Phosphorylase b is a dimer, usually exists in inactive form in the skeletal muscles. Equilibrium exists between an active relaxed (R) state and a less active tense (T). Phosphorylase b favors the T state. The enzyme possesses three domains, the N-terminal domain, glycogen-binding domain and the C-terminal domain.

Application

Phosphorylase b from rabbit muscle is to be used as a marker in SDS-PAGE. Phosphorylase b is used during chemical cross-linking studies as a SDS-PAGE molecular weight standard.

Phosphorylase b from rabbit muscle has been used as a molecular weight marker in 12% polyacrylamide gel for keratinase, and stress-70 protein.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)