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NCTC 135 Medium

Name: NCTC 135 Medium
Brand: SIGMA

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

NACRES:

NA.75

UNSPSC Code:

12171500

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Properties

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO₃: no
L-glutamine: 0.13573 g/L
glucose: 1.0 g/L
phenol red: 0.02 g/L

shipped in

ambient

storage temp.

2-8°C

Description

General description

NCTC 135 Medium is ideal for serum-free growth of mouse L-cells.

Application

NCTC 135 Medium has been used:

to culture Philophthalmus sp. rediae in vitro[1]
to culture Ehrlich ascites mouse tumor cell line
as a culture media for the differentiation of Rcho-1 cells[2]

Preparation Note

Formulated to contain 9.3 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

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This Item	N1140	M7403	M8537
SAFC N3262 NCTC 135 Medium	Sigma-Aldrich N1140 NCTC 109 Medium	Sigma-Aldrich M7403 MCDB 153 Medium	Sigma-Aldrich M8537 MCDB 131 Medium
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form liquid	form powder	form powder
components NaHCO₃: no L-glutamine: 0.13573 g/L glucose: 1.0 g/L phenol red: 0.02 g/L	components glucose: 1.0 g/L phenol red: 0.02 g/L L-glutamine: no NaHCO₃: 2.2 g/L	components NaHCO₃: no L-glutamine: 0.8772 g/L glucose: 1.081 g/L (Dextro) sodium pyruvate: 0.055 g/L phenol red: 0.001242 g/L HEPES: 6.6 g/L (25mM)	components sodium pyruvate: 0.11 g/L phenol red: 0.012 g/L L-glutamine: 1.461 g/L HEPES: no glucose: 1.0 g/L (Dextro)
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
classic media, classical media, FBS, Mouse L-cell culture	mouse L929 cell cultue medium, carcinoma cell line establishment	keratinocye cloning medium, keratinocye long-term culture	HMVEC cloning medium, muscle cell culture medium

Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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