This product is expected to be freely soluble in water. For assay purposes, this material is reconstituted at 1 mg/mL in 20 mM K-phosphate buffer, pH 7.5 containing 2.0 mM MgCl2 and 0.5 mM EDTA-Na3. This product is lyophilized from a solution containing Mg++ and bovine serum albumin as stabilizers.
L9518
Lipase from Pseudomonas sp.
Type XIII, lyophilized powder, ≥15 units/mg solid
Synonym(s):
Extracellular lipase, Triacylglycerol ester hydrolase, lip, Triacylglycerol acylhydrolase, Triacylglycerol lipase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-669-1
MDL number:
Specific activity:
≥15 units/mg solid
Pricing and availability is not currently available.
type
Type XIII
Quality Segment
form
lyophilized powder
specific activity
≥15 units/mg solid
mol wt
~134 kDa
composition
Protein, 40-65% biuret
storage temp.
2-8°C
General description
Research area: Cell Signaling
Lipases are part of the α/β-hydrolase fold superfamily of enzymes and are expressed in various tissues. For instance, hepatic lipases are found in the liver, lipoprotein lipase is located on the vascular endothelial surface, hormone-sensitive lipases are present in adipocytes, and pancreatic lipase is situated in the small intestine.
Lipases are part of the α/β-hydrolase fold superfamily of enzymes and are expressed in various tissues. For instance, hepatic lipases are found in the liver, lipoprotein lipase is located on the vascular endothelial surface, hormone-sensitive lipases are present in adipocytes, and pancreatic lipase is situated in the small intestine.
Isoelectric point : 5.95 -/+0.05
Inhibitors : Hg++, Ag+, ionic detergents
Optimum pH : 7.0 - 9.0
Optimum temperature : 45 - 50oC
pH Stability : pH 7.0 - 9.0 (25oC, 20hr)
Thermal stability : below 55oC (pH 7.0, 10min)
Inhibitors : Hg++, Ag+, ionic detergents
Optimum pH : 7.0 - 9.0
Optimum temperature : 45 - 50oC
pH Stability : pH 7.0 - 9.0 (25oC, 20hr)
Thermal stability : below 55oC (pH 7.0, 10min)
Application
Lipase from Pseudomonas sp. has been used:
- for enzymatic determination of triglyceride in serum when coupled with L-α- glycerophosphate oxidase (G3O-301, G3O-311, G3O-321) and glycerol kinase (GYK-301, GYK-311).
- in a study to assess enzymatic synthesis of biodiesel from palm oil assisted by microwave irradiation. [1]
- in determining the triglyceride content for identifying the role of gut bacteria in host health.[2]
- to determine the effect of lipase on high-density lipoprotein 2 (HDL2) and plasma in vitro.[3]
Biochem/physiol Actions
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Lipase from Pseudomonas was shown to inhibit monocyte chemotaxis in human peripheral blood. [4]It functions by employing chymotrypsin-like hydrolysis, involving a histidine base, a serine nucleophile, and aspartic acid. Lipases are also crucial for lipid transport. Elevated serum lipase levels are often observed in pancreatitis. Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols.
Physical form
Lyophilized powder containing Mg+2, sodium cholate, and bovine serum albumin as stabilizers
Analysis Note
Protein determined by biuret.
Other Notes
One unit will produce 1.0 μmole of glycerol from a triglyceride per min at pH 7.0 at 37 °C in the presence of bovine serum albumin.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥15 units/mg solid | specific activity ≥300 units/mg solid (using olive oil) | specific activity ≥4,000 units/mg solid (using olive oil) | specific activity ≥50,000 units/mg solid |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt ~134 kDa | mol wt - | mol wt - | mol wt - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level - | Quality Level 100 | Quality Level 200 |
| type Type XIII | type - | type - | type - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Could you please give me information on solubility of this enzyme.
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