K2876
Keratanase from Pseudomonas sp.
lyophilized powder
Synonym(s):
Endo-β-galactosidase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
Recommended Products
form
lyophilized powder
packaging
vial of >10 units
storage temp.
−20°C
Unit Definition
One unit will liberate 1.0 μmole of reducing sugar (measured as galactose) from keratan sulfate per hr at pH 7.4 at 37°C.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Kiwamu Yamagishi et al.
The Journal of biological chemistry, 278(28), 25766-25772 (2003-05-07)
Keratan sulfate (KS) is degraded by various enzymes including endo-beta-galactosidase, keratanase, and keratanase II, which are used for the structural analysis of KS. We purified a novel KS hydrolase, endo-beta-N-acetylglucosaminidase, from the cell pellet and conditioned medium of Bacillus circulans
M N Fukuda
Current protocols in molecular biology, Chapter 17, Unit17-Unit17 (2008-02-12)
This overview covers the endo-beta-galactosidases; enzyme is capable of hydrolyzing a wide range of glycoconjugates. Endo-beta-galactosidases from numerous sources are discussed in terms of their substrate specificities and substrates, as well as their practical research applications.
Takeomi Murata et al.
Biochimica et biophysica acta, 1722(1), 60-68 (2005-02-18)
Enzymatic synthesis of GlcNAc-terminated poly-N-acetyllactosamine beta-glycosides GlcNAcbeta1,3(Galbeta1,4GlcNAcbeta1,3)(n)Galbeta1,4GlcNAcbeta-pNP (n=1-4) was demonstrated using a transglycosylation reaction of Escherichia freundii endo-beta-galactosidase. The enzyme catalyzed a transglycosylation reaction on GlcNAcbeta1,3Galbeta1,4GlcNAcbeta-pNP (1), which served both as a donor and an acceptor, and converted 1 into
N Yonezawa et al.
Molecular reproduction and development, 70(2), 222-227 (2004-12-01)
The zona pellucida (ZP) surrounding the mammalian oocyte is composed of three glycoprotein components (ZPA, ZPB, and ZPC). Mammalian sperm bind to carbohydrate chains of a ZP glycoprotein in the initial phase of fertilization. Sperm-ligand carbohydrate chains have been characterized
Saeed Akhtar et al.
Investigative ophthalmology & visual science, 49(6), 2424-2431 (2008-06-03)
To evaluate the use of neoepitope monoclonal antibody BKS-1, which recognizes keratanase-generated keratan sulfate (KS) stubs on keratan sulfate proteoglycans in human cornea, limbus, and sclera. BKS-1 specifically recognizes a keratanase-generated neoepitope [N-acetyl-glucosamine-6-sulfate (GlcNAc-6-S)] at the nonreducing terminal of corneal
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