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5-(Iodoacetamido)fluorescein

Name: 5-(Iodoacetamido)fluorescein
Brand: SIGMA

≥90% purity (HPLC), powder

Synonym(s):

5-IAF

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About This Item

Empirical Formula (Hill Notation):

C₂₂H₁₄INO₆

CAS Number:

63368-54-7

Molecular Weight:

515.25

UNSPSC Code:

12171500

PubChem Substance ID:

329815467

NACRES:

NA.47

Beilstein/REAXYS Number:

6543244

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Properties

Product Name

5-(Iodoacetamido)fluorescein, ≥90% (HPLC)

Quality Segment

200

assay

≥90% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to orange

solubility

DMSO: 5 mg/mL

fluorescence

λ_ex 492 nm; λ_em 515 nm (Mercaptoethanol adduct), λ_ex 493 nm; λ_em 522 nm (pH 9.2)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(NC(=O)CI)ccc45)c1

InChI

1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27)

InChI key

UATCLPJEZJKNHE-UHFFFAOYSA-N

Description

General description

5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative.

Application

5-(Iodoacetamido)fluorescein is used for the synthesis of fluorescently labeled organelles (nuclei and nuclear matrices), proteins and peptides that include enzymes.

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This Item	D0531	F9551	F3651
Sigma-Aldrich I9271 5-(Iodoacetamido)fluorescein	Sigma-Aldrich D0531 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride	Sigma-Aldrich F9551 Fluorescein-5-EX N-hydroxysuccinimide ester	Sigma-Aldrich F3651 Fluorescein 5(6)-isothiocyanate
technique(s) titration: suitable	technique(s) -	technique(s) titration: suitable	technique(s) titration: suitable
color yellow to orange	color yellow to orange	color yellow to orange	color yellow to orange
fluorescence λ_ex 492 nm; λ_em 515 nm (Mercaptoethanol adduct), λ_ex 493 nm; λ_em 522 nm (pH 9.2)	fluorescence λ_ex 495 nm; λ_em 516 nm	fluorescence λ_ex 511-519 nm	fluorescence -
form powder	form powder	form powder	form powder
solubility DMSO: 5 mg/mL	solubility -	solubility DMSO or DMF: soluble	solubility acetone: 1 mg/mL, clear, light yellow to yellow
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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