I1645

Interleukin-8 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-8, CXCL8, neutrophil chemotactic factor
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00131404

Properties

• biological source: human
• Quality Segment: 100
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: monomer 8.9 kDa ((77 aa))
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg (LAL test)
• UniProt accession no.: P10145
• storage temp.: −20°C
• Gene Information: human ... IL8(3576)

Description

Application

Interleukin-8 (IL-8) human has ben used to:

• measure neutrophil chemotoxis[1][2]
• study the uptake of apoptotic cells by meningothelial cells[3]
• study the effect of IL-8 on expression and secretion of urocortin-1 by human umbilical vein endothelial cells[4]

Biochem/physiol Actions

Interleukin-8 (IL-8) was formerly called monocyte-derived neutrophil chemotactic factor. It belongs to the chemokine α or C-X-C family. Like other members of this family, the mature form of IL-8 has four cysteine residues and the first two cysteine residues are separated by glutamine. IL-8 shows chemotactic activity in vitro for T cells, neutrophils and basophils.[5] Mutation in IL-8 can increase risk to acute pancreatitis.[6]

Interleukin-8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the chemokine α or C-X-C family. The mature form of IL-8 has 4 cysteine residues, as do the other members of the chemokine family and the first two cysteine residues are separated by glutamine. Mature human IL-8 consists of 72 amino acids with a molecular mass of 8.9 kDa. IL-8 exhibits chemotactic activity in vitro for T cells, basophils, as measured by enzymes including myeloperoxidase, α-mannosidase and β-glucuronidase. IL-8 is an angiogenic factor.

Physical form

Lyophilized from a 0.2 μm filtered solution containing 10 mM sodium phosphate, pH 7.5

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)