H4652

HMG-1 Peptide

≥90% (SDS-PAGE), lyophilized powder, Histidine-tagged, human recombinant, expressed in E. coli

• Synonym(s): Amphoterin, HMGB1, High-Mobility-Group protein
• UNSPSC Code: 12352202
• PubChem Substance ID: 24895651
• NACRES: NA.42
• MDL number: MFCD03457085

Properties

• Product Name: HMG-1 human, lyophilized powder, ≥90% (SDS-PAGE), Histidine-tagged, recombinant, expressed in E. coli
• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 25 kDa
• UniProt accession no.: P09429
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: COC(=O)CCCCCCCCCCCCCCO
• InChI key: WQCYAHKAJFZVCO-UHFFFAOYSA-N
• Gene Information: human ... HMGB1(3146)

Description

General description

215 amino acids; comprises two homologous HMG boxes, rich in basic amino acids, and an acidic tail at the carboxy-terminus

HMG-1 (high mobility group protein) is a nuclear protein of molecular weight 30kDa. It is secreted from macrophages, monocytes and necrotic cells.[1] It is ubiquitously expressed. The protein has basic HMG (high mobility group) box domains and an acidic carboxyl-terminus.[2]

Application

HMG-1 (high-mobility-group protein) human has been used:

• to prepare a standard curve for measuring HMG1 using ELISA (enzyme-linked immunosorbent assay) test[3][3]
• to study the apoptotic ratios of Lewis cells in presence of exogenous HMG-1[4]
• to study the changes in Th (T helper)-17 cell expansion in the presence of HMG-1 under in vitro conditions[5]
• for the migration assay of GM7373 cells[6]

Biochem/physiol Actions

HMG-1 (high mobility group protein) is a non-histone chromosomal protein, which promotes the sequence specific DNA attachment of proteins. HMG1 binds DNA in a non-sequence specific manner.[7] It is involved in DNA replication, transcription and repair. In addition, it is up-regulated in cancers and is associated with cellular growth, vasculogenesis and apoptosis regulation.[8] In the presence of infection or injury, HMG1 is secreted and mediates inflammation.[9] It is associated with the pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA), inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE).[1]

Physical form

Lyophilized powder containing HEPES buffer salts, pH 7.8, NaCl, EDTA, Triton X-100, Leupeptin and AEBSF.

Analysis Note

Tested in DNA retardation assay

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves
• hcodes: H318,H410,EUH430
• pcodes: P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1