GE29-0510-21

HisTrap^™ High Performance

Cytiva 29-0510-21, pack of 1 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• packaging: pack of 1 mL
• manufacturer/tradename: Cytiva 29-0510-21
• availability: not available in North America
• parameter: flow rate; 4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature); 42 psi (H₂O at room temperature.)
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium)
• capacity: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(H2O at room temperature.); ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Description

General description

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Application

HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

Features and Benefits

• High binding capacity, at least 40 mg/ml chromatography medium.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Negligible Ni²⁺ leakage
• Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Legal Information

HisTrap is a trademark of Cytiva

Safety Information

• Pictograms: GHS09
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids