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G4671

Sigma-Aldrich

Anti-GATA1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-ERYF1, Anti-Erythroid transcription factor 1, Anti-Globin transcription factor 1

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About This Item

MDL number:
MFCD02096392
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

human, rat, mouse

concentration

~1 mg/mL

technique(s)

immunohistochemistry: 1:100-1:250
western blot: 1:500-1:1,000

UniProt accession no.

P15976

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... GATA1(2623)
mouse ... Gata1(14460)
rat ... Gata1(25172)

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Immunogen

synthetic peptide corresponding to residues 211-225 of human GATA1.

Application

Anti-GATA1 antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:500 to 1:1000 and for immunohistochemistry at a working dilution of 1:100 to 1:250.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

GATA1 belongs to the GATA family of transcription factors. The encoded protein regulates erythroid development and megakaryocyte differentiation. It binds to the consensus sequence 5′-[AT]GATA[AG]-3′ within the regulatory region. Defects in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Reduced levels of GATA1 may cause Diamond-Blackfan anemia.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C D Trainor et al.
Nature, 343(6253), 92-96 (1990-01-04)
Vertebrate erythroid cells contain a tissue-specific transcription factor referred to as Eryf 1 (ref. 1), GF-1 (ref. 2) or NF-E1 (ref. 3), for which binding sites are widely distributed in the promoters and enhancers of the globin gene family, and
Luciana M Hollanda et al.
Nature genetics, 38(7), 807-812 (2006-06-20)
Acquired somatic mutations in exon 2 of the hematopoietic transcription factor GATA-1 have been found in individuals with Down syndrome with both transient myeloproliferative disorder and acute megakaryoblastic leukemia. These mutations prevent the synthesis of the full-length protein but allow
K E Nichols et al.
Nature genetics, 24(3), 266-270 (2000-03-04)
Haematopoietic development is regulated by nuclear protein complexes that coordinate lineage-specific patterns of gene expression. Targeted mutagenesis in embryonic stem cells and mice has revealed roles for the X-linked gene Gata1 in erythrocyte and megakaryocyte differentiation. GATA-1 is the founding
Leif S Ludwig et al.
Nature medicine, 20(7), 748-753 (2014-06-24)
Ribosomal protein haploinsufficiency occurs in diverse human diseases including Diamond-Blackfan anemia (DBA), congenital asplenia and T cell leukemia. Yet, how mutations in genes encoding ubiquitously expressed proteins such as these result in cell-type- and tissue-specific defects remains unknown. Here, we
Fengyun Sun et al.
Development (Cambridge, England), 137(10), 1699-1707 (2010-05-01)
The ENU-induced repro8 mutation was identified in a screen to uncover genes that control mouse gametogenesis. repro8 causes male-limited infertility, with failure of spermatocytes to exit meiotic prophase via the G2/MI transition. The repro8 mutation is in the Eif4g3 gene
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