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F7296
Fructosyl-Amino Acid Oxidase from Corynebacterium sp.
recombinant, expressed in E. coli, lyophilized powder, ≥0.45 units/mg protein
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About This Item
Specific activity:
≥0.45 units/mg protein
Recombinant:
expressed in E. coli
Pricing and availability is not currently available.
recombinant
expressed in E. coli
Quality Segment
form
lyophilized powder
specific activity
≥0.45 units/mg protein
mol wt
~88 kDa by electrophoresis
storage temp.
−20°C
General description
Enzyme Commission (E.C.) 1.5.3.x
Fructosyl amino acid oxidase [fructosyl-a-l-amino acid:oxygen oxidoreductase] is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide.[1]
Application
Fructosyl-amino acid oxidase can be used to detect the levels of glycated proteins, which are markers for diabetes mellitus.
Fructosyl-Amino Acid Oxidase from Corynebacterium sp has been used in glycated haemoglobin HbA1c detection in blood samples using quartz crystal microbalance (QCM) based detection.
Biochem/physiol Actions
Fructosamines are formed when glucose is condensed amino group of amino acids or proteins.[2] Fructosamine oxidases (FAOX) catalyze the oxidative deglycation of low molecular weight fructosamines. Fructosyl amino acid oxidase catalyzes the oxidation of the C-N bond linking the C1 of the fructosyl moiety and the nitrogen of the amino group of fructosyl amino acids.[3]
Suitable for the determination of fructosyl-L -amino acid.
Fructosyl-Amino Acid Oxidase (FAOD) comprises of FAD-binding motifs and is classified into three types based on substrate specificity. The engineered Corynebacterium Fructosyl-Amino Acid Oxidase is stable at 45°C and could be exploited for the development of glycated protein biosensing system[3] and glycated hemoglobin HbA1c measurements. FAOD shares sequence homology with fructosyl peptide oxidase and both are effective on α-fructosyl substrates.
Other Notes
One unit will produce 1.0 μmole of hydrogen peroxide per minute at pH 8.0 at 37 °C.
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This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, lyophilized powder, ≥0.45 units/mg protein | description recombinant, expressed in E. coli ≥12 units/mg protein | description lyophilized powder, 25-50 units/mg solid | description lyophilized powder, ≥60 units/mg protein |
| specific activity ≥0.45 units/mg protein | specific activity ≥12 units/mg protein | specific activity 25-50 units/mg solid | specific activity ≥60 units/mg protein |
| form lyophilized powder | form powder | form lyophilized powder | form lyophilized powder |
| recombinant expressed in E. coli | recombinant expressed in E. coli ≥12 units/mg protein | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~88 kDa by electrophoresis | mol wt - | mol wt 44 kDa | mol wt 245 kDa |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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